HiTSeeker D5 Dopamine Receptor Cell Line

Reference: P30122

HiTSeeker D5 Dopamine Receptor Cell Line

HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker D5 Dopamine Receptor Cell Line contains HEK293 cells stably expressing DRD5 with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.

Innoprot HiTSeeker DRD5 Cell Line allows to assay compounds, or analyze their capability to modulate DRD5. When a ligand binds to the DRD5, it activates a G protein, which in turn, triggers a cellular response mediated by cAMP. HiTSeeker DRD5 cell line has been validated measuring cAMP concentration changes in the cytosol upon activation with Dopamine. The high reproducibility of this assay allows monitoring DRD5 receptor activation process in High Throughput Screening.

Size/Quantity: 2 vials containing 3×106 cells / vial


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Dopamine receptors play important roles in many neurological processes, including motivation, pleasure, cognition, memory, learning, and fine motor control; as well as modulation of neuroendocrine signaling. Abnormal dopamine receptor signaling and dopaminergic nerve function occurs in several neuropsychiatric disorders. Thus, dopamine receptors are common neurologic drug targets. The D1 and D5 receptors have a high degree of structural homology and few ligands are available that can distinguish between them as yet; however there are a number of ligands that are selective for D1/5 over the other dopamine receptors. The recent development of a selective D5 antagonist has allowed the action of D1-mediated responses to be studied in the absence of a D5 component, but no selective D5 agonists are yet available. HiTSeeker D5 Dopamine Receptor Cell Line allows the measurement of the activity of ligands, such as Dopamine.

  • Reference: P30122
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice