The H1 receptor is a histamine receptor belonging to the family of rhodopsin-like G-protein-coupled receptors. Histamine H1 receptor is present in several tissues such as central nervous system, cardiovascular system, smooth muscles, or lymphocytes. In vascular endothelial cells, stimulation of histamine H1 receptors lead to the synthesis and release of several neurochemicals and neuromodulators; such as prostacyclin, platelet–activating factor, and nitric oxide (NO). In addition, stimulation of the H1 receptor causes changes in vascular permeability, particularly the postcapillary venule as a result of endothelial cell contraction. Antihistamines, which act on this receptor, are anti-allergy drugs. HiTSeeker Histamine H1 Receptor Cell Line from Innoprot allows the measurement of the activity and potency of ligands; such as Histamine.
HiTSeeker Histamine H1 Receptor Cell Line
HiTSeeker Histamine H1 Receptor Cell Line
HiTSeeker are cell lines stably expressing label-free GPCRs, also known as seven-(pass)-transmembrane domain receptors. Each vial of HiTSeeker Histamine H1 Receptor Cell Line contains U2OS cells stably expressing H1 Receptor with no tag. HiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual.
Innoprot HiTSeeker Histamine H1 Receptor Cell Line allows to assay compounds, or analyze their capability to modulate H1 receptor. When a ligand binds to the BB2 receptor, it activates a G protein, which in turn, triggers a cellular response mediated by calcium. HiTSeeker H1 Receptor cell line has been validated measuring calcium concentration changes in the cytosol upon activation with Histamine. The high reproducibility of this assay allows monitoring H1 Receptor activation process in High Throughput Screening.
Size/Quantity: 2 vials containing 3×106 cells / vial
5.250,00€
- Product Description
- Specifications
- Characterization
- Similars
- Publications
- Technical Resources & Protocols
- Reference: P30183
- Size/Quantity: 2 vials containing 3x106 cells / vial
- Product Use: For research use only
- Shipping Conditions: Dry Ice
