Human Gastric fibroblasts (HGF), which are present in the stomach, are mesenchymal cells from the embryonic mesoderm. Fibroblasts secrete a non-rigid extracellular matrix that is both rich in type I and/or type III collagen. They are responsible of the synthesis of extracellular matrix in connective tissues and play a central role in wound healing. In addition to their role in wound healing, human gastric fibroblasts play an important role in the development and progression of gastric carcinoma. HGF are a useful model for examining the underlying mechanisms that cause gastric carcinoma; so they may provide novel insights for developing new cancer drug therapies.