Iris is a pigmented disk which controls the size of the pupil and the amount of light reaching the retina. It consists of the anterior limiting layer, the stroma, the dilator muscle layer, and the posterior pigmented epithelium. The main function of Human Iris Fibroblasts is to maintain the structural integrity of the connective tissue; but also to aid in tissue repair and remodeling. Under disease conditions, such as with rubeosis iridis, neovascularization can occur on the anterior surface of the iris and result in fibrosis. Patients with diabetes and diabetic retinopathy are at a higher risk of developing rubeosis iridis. Human Iris Fibroblasts will surely continue to provide an in vitro model in the pathophysiological studies of eye vascular diseases.