HypoThermosol is an optimized hypothermic (2-8°C) preservation media that enables improved and extended preservation of cells, tissues and organs. HypoThermosol is uniquely formulated to address the molecular-biological response of cells during the hypothermic preservation process. HypoThermosol includes key ions at concentrations that balance the intracellular state at hypothermic temperatures. Additional components include pH buffers, energy substrates, free radical scavengers, and osmotic/oncotic stabilizers.