Oligodendrocyte Precursor Cell Differentiation Medium (OPCDM) is a complete medium designed for the differentiation of oligodendrocyte precursor cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (1%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes the differentiation of normal human oligodendrocyte precursor cells in vitro.