Vascular endothelial cells contribute to the maintenance of vascular homeostasis. Vascular endothelial cells produce and secrete activators and inhibitors of the coagulation and fibrinolysis system. In addition, they mediate the adhesion and aggregation of blood platelets. Endothelial cells also release molecules that regulate cell proliferation and control vessel wall tone. Human umbilical vein endothelial cells (HUVEC) are the type of cells most used in the study of endothelial cell processes. HUVEC have a “cobblestone” morphology, show positive staining for vWF/Factor VIII and CD-31, and the ability to take up acetylated low-density lipoprotein. Cells pretreated with IL-1 or TNF-alpha also selectively express E-selectin and VCAM.
About turboFP602 protein:
TurboFP602 is a red-shifted variant of the red fluorescent protein TurboRFP from sea anemone Entacmaea quadricolor [Merzlyak et al., 2007]. TurboFP602 possesses true-red fluorescence (with excitation/emission maxima at 574/602 nm, respectively), optimal for detection via most popular filter sets, and is also easily distinguished from background signals. This red frluorescent protein exhibits fast maturation and high pH stability.
TurboFP602 is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.