Green Fluorescent NIH/3T3 Cell Line are suitable for both, in vitro and in vivo experimentation.
About NIH/3T3 Cell line:
NIH 3T3 mouse embryonic fibroblast cells come from a cell line isolated in 1962. 3T3 refers to the cell transfer and inoculation protocol for the line, and means “3-day transfer, inoculum 3 x 105 cells”. Using this protocol, the immortal cell line begins to thrive and stabilize in cell culture after about 20-30 generations of in vitro growth. The scientists who first cultured this cell line, obtained the cells from desegregated NIH Swiss mouse embryo fibroblasts. The cell line has since become a standard fibroblast cell line. 3T3 cells are sensitive to sarcoma virus focus formation, as well as leukemia virus.
About turboFP602 protein:
TurboFP602 protein is a red shifted variant of the red fluorescent protein TurboRFP from sea anemone Entacmaea quadricolor [Merzlyak et al., 2007]. It possesses true-red fluorescence (excitation/ emission max = 574/ 602 nm) that is optimal for detection via most popular filter sets. TurboFP602 exhibits fast maturation and also high pH stability. TurboFP602 is very useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.