TDP-43 Stress Granules Assay Cell Line
TDP-43 Stress Granules Assay Cell Line
TDP-43 Stress Granules Assay Cell Line

TDP-43 Stress Granules Assay Cell Line

Reference: P30710

TDP-43 Stress Granules Assay Cell Line

Innoprot has developed a novel fluorescence cell-based assay cell line for High Content Screening that allows the quantification of pathological TDP43 globs into the nucleus and cytosol. This model consists in a U2OS cell line stably expressing inducible fluorescent TDP-43. U2O2 cells stably expressing human TAR DNA-binding protein 43 (TDP-43) must be induced with IPTG 5mM during 48h to produce the hTDP43-tGFP protein.

Innoprot offers this cell line as a “stable cell line” in cryopreserved vials. Each vial of U2OS cell line stably expressing fluorescent TDP-43 contains 3 million cells and Innoprot provides 2 vials of cells with each order. These cells proliferate in vitro and they are guaranteed to further expand for more than 30 passages following the instructions provided in the technical sheet.

Size/Quantity: 2 vials containing 3×106 cells / vial

9.995,00€

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Amyotrophic lateral sclerosis (ALS) is one of the most common degenerative disease of the motor neuron system. The TDP-43 pathology seems to be a dominant type of pathology across sporadic ALS types. Our TDP-43 Stress Granules Assay cell line allows the quantification of pathological TDP43 globs into the nucleus and cytosol. This model also permits to monitor the TDP-43 protein distribution in living cells; studying the protein localization pattern in the space and time, and quantifying the fluorescence aggregation inside the cells.

To perform the assay, we induce fluorescent TDP-43 expression adding IPTG to the cell culture medium. After TDP-43 expression induction and during the assay, we incubate the cells with test compounds and Sodium Arsenite to induce the TDP-43 aggregation. Finally, we quantify TDP43-tGFP nuclear globs using the BD Pathway HCS Reader and Attovision Compartimentalization Software.

 

Applications:

  • TDP-43 Stress Granules Assay cell line allows the quantification of pathological TDP43 globs.
  • This model permits to evaluate the TDP-43 protein distribution in living cells studying the protein localization pattern in the space and time.
  • This model provides a strategy to evaluate drugs without cell permeability.
  • This cellular model have been adapted to HCS analyses based on image algorithms to test cytosolic and nuclear globs generation process.
  • Reference: P30710
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • DNA Accesion Number: GenBank NM_000176
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice
    ALS model | TDP-43