MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD H1 Histamine Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human H1 Histamine Receptor with no tag
Calcium Nomad Biosensor (Green Fluorescence)
β-arrestin Nomad Biosensor (Red Fluorescence)
MPXNOMAD H1 Histamine Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to H1 receptor a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor both G-protein pathways. Each vial of MPXNOMAD NK2 Tachykinin Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human NK2 Receptor with no tag
Calcium Nomad Biosensor (Green Fluorescence)
cAMP Nomad Biosensor (Red Fluorescence)
MPXNOMAD NK2 Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; both G proteins by Ca2+ & cAMP flux, hence allowing biased activity studies. When an agonist binds to NK2 Receptor two G proteins are activated which, in turn, triggers a cellular response mediated by calcium and cAMP. This leads to an increase of fluorescence intensity of both cAMP and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD NTS1 Neurotensin Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human NTS1 Neurotensin Receptor with no tag
Calcium Nomad Biosensor (Green Fluorescence)
β-arrestin Nomad Biosensor (Red Fluorescence)
MPXNOMAD NTS1 Neurotensin Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to NTS1R a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD PAR2 Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human PAR2 Receptor with no tag
Calcium Nomad Biosensor (Green Fluorescence)
β-arrestin Nomad Biosensor (Red Fluorescence)
MPXNOMAD PAR2 Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to PAR2 Receptor a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD α1A-adrenoceptor Cell Line contains HEK293 cells stably expressing the following constructs:
human α1A-adrenoceptor with no tag
Calcium Nomad Biosensor (Green Fluorescence)
β-arrestin Nomad Biosensor (Red Fluorescence)
MPXNOMAD α1A-adrenoceptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Calcium flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to ADRA1A a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD α1B-adrenoceptor Cell Line contains HEK293 cells stably expressing the following constructs:
human α1B-adrenoceptor with no tag
Calcium Nomad Biosensor (Green Fluorescence)
β-arrestin Nomad Biosensor (Red Fluorescence)
MPXNOMAD α1B-adrenoceptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Calcium flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to ADRA1B a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
PKANOMAD Cells are cell lines stably co-expressing tag-free GPCRs and PKA Nomad Biosensor to monitor G-protein modulation by PKA activation. Each vial of PKANOMAD Muscarinic M4 Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human Muscarinic M4 Receptor (M4R) with no tag
PKA Nomad Biosensor (Red Fluorescence)
PKANOMAD Muscarinic M4 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gi/Go involving receptor activation. When an agonist binds to M4 Receptor, the Gi/Go protein is activated which, in turn, triggers a cellular response mediated by cAMP via PKA activation. This leads to an increase of red fluorescence intensity of PKA biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
β-ArNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and β-arrestin Nomad Biosensor to GPCR β-arrestin modulation. Each vial of β-ArNOMAD CCK2 Cholecystokinin Receptor Cell Line contains U2OS cells stably expressing the following constructs:
human CCK2 Cholecystokinin Receptor with no tag
β-arrestin Nomad Biosensor (Green Fluorescence)
β-ArNOMAD CCK2 Cholecystokinin Receptor Cell Line allows to assay compounds analyzing β-arrestin recruitment. When an agonist binds to CCK2R a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. Due to the presence of ß-Arrestin Nomad Biosensor, this recruitment leads to an increase of green fluorescence intensity. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
The ACE2 cell line from Innoprot has been developed stably transfecting HEK293 cell line with a angiotensin I converting enzyme 2 (ACE2) protein expression plasmid. ACE2 HEK293 cell line provides consistent levels of expression of human ACE2 protein in cells surface. Each vial of cells contains more than 3 million viable cells stably expressing ACE2 with hygromycin resistance gene.
ACE2 HEK293 Cell line is ready to use in cell-based assay applications, such as Screening for ACE2 inhibitors. This stably transfected clonal cell line provides consistent levels of expression, which helps simplify the interpretation of results. ACE2 HEK293 Cell line also allow to stablish in vitro models for High Throughput and High Content Screening.
Adipocyte Medium Kit is a complete medium designed which contains all of the components necessary to support lipid accumulation in the maturing adipocytes. Each Adipocyte Medium Kit consists of the next components:
A novel family of stable cell lines have been developed through stable transfection with different fluorescent-α-synuclein. TagGFP2 and tRFP- α-synuclein cell lines are stably-transfected and it is ready to use in cell-based assay applications. These stably transfected cell linew provide consistent levels of expression, which helps to simplify the interpretation of the results. These cell lines are intended to be used as an “in vitro” models for research studies.
Cell Lines available
SH-SY5Y cell line stably expressing green fluorescent alpha – synuclein
SH-SY5Y cell line stably expressing red fluorescent alpha – synuclein
HEK293 cell line stably expressing green fluorescent alpha – synuclein
HEK293 cell line stably expressing red fluorescent alpha – synuclein
U2OS cell line stably expressing green fluorescent alpha – synuclein
All vials of the different stable cell line contains 3 million cells. It is guaranteed that these cells will expand in vitro following the cell culture manual
Alveolar Epithelial Cell Medium Kit is a complete medium designed for optimal growth of normal primary alveolar epithelial cells in vitro. Each Alveolar Epithelial Cell Medium Kit consists of the next components:
APP Processing Assay Cell Line is an Alzheimer’s Disease in vitro model to identify Aβ peptide formation inhibitors such as secretase activity inhibitors. It consists in MDCK cell line stably expressing green fluorescent human amyloid precursor protein (APP). To perform the assay, cells are incubated with test compounds during 48-72 hours to evaluate their effects on APP Processing. After incubation, nucleus could be stained with DAPI and retained fluorescent APP vesicles are detected by fluorescence using image analysis algorithms.
Innoprot offers this assay as a “stable cell line” in cryopreserved vials. Each vial of MDCK cell line stably expressing human tGFP-APP contains 3 million cells and Innoprot provides 2 vials of cells with each order. It is guaranteed that these cells will expand in more than 30 passages following the cell culture manual.
Astrocyte Medium Kit is a complete medium designed for optimal growth of normal primary astrocytes in vitro. Each Astrocyte Medium Kit consists of the next components:
Bovine Brain Microvascular Endothelial cells (BBMEC) provided by Innoprot are isolated from bovine brain. BBMEC are cryopreserved at passage one and delivered frozen. Each vial of Bovine Brain Microvascular Endotheilal Cells contains more than 500.000 viable cells. They are guaranteed to further expand for 10 population doublings following the instructions provided in the technical sheet.
Innoprot also offers optimized medium and reagents for the growth of Bovine Brain Microvascular Endothelial Cells which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro BBMEC culture.
Bovine Plasma Fibronectin is purified from bovine plasma with affinity chromatography and supports cell adhesion and spreading. Recommended for use as a cell culture substratum at 1-5 µg/cm2. Optimal concentration depends on cell type.
