Green Fluorescent Human Hepatic Stellate Cells (TTFLUOR HHSteC) provided by Innoprot are isolated from human healthy liver and transfected with tGFP. TTFLUOR HHSteC are cryopreserved at secondary culture and delivered frozen. Each vial of Green Fluorescent Human Hepatic Stellate Cells contains more than 500.000 million viable cells. They are guaranteed to further expand for 10 population doublings following the instructions provided in the technical sheet.
Innoprot also offers optimized medium and reagents for the growth of TTFLUORHuman Hepatic Stellate Cells which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro HHSteC culture.
Green Fluorescent Human Primary Astrocytes (TTFLUOR HAs) provided by Innoprot are isolated from human brain cortex and transfected with tGFP. TTFLUOR HAs are cryopreserved at secondary culture and delivered frozen. Each vial of Green Fluorescent Human Astrocytes contains more than 500.000 million viable cells. They are guaranteed to further expand for 10 population doublings following the instructions provided in the technical sheet.
Innoprot also offers optimized medium and reagents for the growth of Green FluorescentHuman Primary Astrocytes which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro TTFLUOR HAs culture.
Green Fluorescent Human Umbilical Vein Endothelial Cells (TTFLUOR HUVEC) provided by Innoprot are isolated from healthy human umbilical vein. TTFLUOR HUVEC are cryopreserved at passage one and delivered frozen. Each vial of TTFLUOR HUVEC contains more than 1 million viable cells. They are guaranteed to further expand for 10 population doublings following the instructions provided in the technical sheet.
Innoprot also offers optimized medium and reagents for the growth of TTFLUOR HUVEC which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro HUVEC culture.
Green Fluorescent Immortalized Human Aortic Endothelial Cell Line (IM-HAEC-tGFP) provided by Innoprot has been developed by immortalizing primary human aortic endothelial cells with SV40 Large T antigen and transfected with tGFP.
Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 3rd passage while the SV40‐tranduced cells go beyond 30 passages.
Each vial of IM-HAEC-tGFP contains more than 1 million viable cells. Innoprot also offers optimized medium and reagents for the growth of IM-HAEC-tGFP which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro IM-HAEC-tGFP culture.
Green Fluorescent Immortalized Human Brain Microvascular Endothelial Cells (IM-HBMEC-tGFP) provided by Innoprot have been developed by immortalizing primary human brain endothelial cells with SV40 Large T antigen and transfected with tGFP.
Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 3rd passage while the SV40‐tranduced cells go beyond 30 passages.
Each vial of IM-HBMEC-tGFP contains more than 1 million viable cells. Innoprot also offers optimized medium and reagents for the growth of IM-HBMEC-tGFP which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro IM-HBMEC-tGFP culture.
Green Fluorescent Immortalized Human Dermal Microvascular Endothelial Cells (IM-HDMEC-tGFP) provided by Innoprot have been developed by immortalizing primary human dermal endothelial cells with SV40 Large T antigen and transfecting with tGFP.
Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 3rd passage while the SV40‐tranduced cells go beyond 30 passages.
Each vial of IM-HDMEC-tGFP contains more than 1 million viable cells. Innoprot also offers optimized medium and reagents for the growth of IM-HDMEC-tGFP which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro IM-HDMEC-tGFP culture.
Green Fluorescent Immortalized Human Astrocytes (IM-HA-tGFP) provided by Innoprot have been developed by immortalizing primary human astrocytes with SV40 Large T antigen and stably transfected with tGFP.
Immortalized cells were controlled passaging side by side with the primary cells. These primary cells go into senescence after the 3rd passage while the SV40‐tranduced cells go beyond 30 passages.
Each vial of Green Fluorescent Immortalized Human Astrocytes contains more than 1 million viable cells. Innoprot also offers optimized medium and reagents for the growth of IM-HA-tGFP which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro IM-HA-tGFP culture.
Green Fluorescent Immortalized Human Microglia (IM-HM-tGFP) provided by Innoprot have been developed by immortalizing primary human microglia with SV40 Large T antigen and stably transfected with tGFP.
Immortalized cells were controlled passaging side by side with the primary cells. These primary cells go into senescence after the 2nd passage while the SV40‐tranduced cells go beyond 30 passages.
Each vial of Green Fluorescent Immortalized Human Microglia contains more than 1 million viable cells. Innoprot also offers optimized medium and reagents for the growth of IM-HM-tGFP which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro IM-HM-tGFP culture.
Green Fluorescent Jurkat Cell Line (aka LINTERNA Jurkat) has been developed through stable transfection of Jurkat cell line with tGFP. This cell line expresses green fluorescent protein gene sequences as free cytoplasmatic proteins. Each vial of cells contains more than 3 million viable cells stably expressing tGFP with G418 resistance gene:
LINTERNA Jurkat Cell line is ready to use in cell-based assay applications. This stably transfected clonal cell line provides consistent levels of expression, which helps simplify the interpretation of results. Green Fluorescent Jurkat Cell Line also allows to stablish in vitro models for HIgh Throughput and High Content Screening.
Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent Kappa Opioid Receptor Cell Line contains U2OS cells stably expressing KOR Receptor tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.
Innoprot Green Fluorescent Kappa Opioid Receptor Cell Line allows to assay compounds, or analyze their capability to modulate KOR Receptor activation and the following redistribution process inside the cells. When a ligand binds to the KOR Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent Kappa Opioid Receptor redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring KOR Receptor redistribution process in High Throughput Screening.
Green Fluorescent LS174T Cell Line (aka LINTERNA LS174T) has been developed through stable transfection of LS174T cell line with tGFP. This cell line expresses green fluorescent protein gene sequences as free cytoplasmatic proteins. Each vial of cells contains more than 3 million viable cells stably expressing tGFP with G418 resistance gene:
LINTERNA LS174T Cell line is ready to use in cell-based assay applications. This stably transfected clonal cell line provides consistent levels of expression, which helps simplify the interpretation of results. Green Fluorescent LS174T Cell Line also allow to stablish in vitro models for HIgh Throughput and High Content Screening.
Green Fluorescent GPCR cell lines from Innoprot allows to perform GPCR internalization assays. Each vial of Green Fluorescent M1 Muscarinic Acetylcholine Receptor Cell Line contains CHO-K1 cells stably expressing M1 Receptor tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual.
Innoprot Green Fluorescent M1 Muscarinic Acetylcholine Receptor Cell Line allows to assay compounds, or analyze their capability to modulate M1R activation and the following redistribution process inside the cells. When a ligand binds to the M1 Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent M1 Receptor redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring M1 Muscarinic Acetylcholine Receptor redistribution process in High Throughput Screening.
Green Fluorescent MDA-MB-231 Cell Line (aka LINTERNA MDA-MB-231) has been developed through stable transfection of MDA-MB-231 cell line with tGFP. This cell line expresses green fluorescent protein gene sequences as free cytoplasmatic proteins. Each vial of cells contains more than 3 million viable cells stably expressing tGFP with puromycin resistance gene:
LINTERNA MDA-MB-231 Cell line is ready to use in cell-based assay applications. This stably transfected clonal cell line provides consistent levels of expression, which helps simplify the interpretation of results. Green Fluorescent MDA-MB-231 Cell Line also allows to stablish in vitro models for HIgh Throughput and High Content Screening.
Green Fluorescent MDCK Cell Line (aka LINTERNA MDCK) has been developed through stable transfection of MDCK cell line with tGFP. This cell line expresses green fluorescent protein gene sequences as free cytoplasmatic proteins. Each vial of cells contains more than 3 million viable cells stably expressing tGFP with G418 resistance gene:
LINTERNA MDCK Cell line is ready to use in cell-based assay applications. This stably transfected clonal cell line provides consistent levels of expression, which helps simplify the interpretation of results. Green Fluorescent MDCK Cell Line also allow to stablish in vitro models for HIgh Throughput and High Content Screening.
A novel green fluorescent U2OS cell line has been developed through stable transfection with human mHTT-92Q (exon 1) under inducible promoter. Green Fluorescent mHTT-92Q (exon 1) inducible cell line is stably-transfected and it is ready to use in cell-based assay applications. This stably transfected cell line provides consistent levels of expression by adding IPTG 1mM, which helps to simplify the interpretation of the results. This cell line is intended to be used as an “in vitro” model for research studies.
Each vial of Green Fluorescent mHTT-92Q (exon 1) inducible cell line contains 3 million cells. It is guaranteed that these cells will expand in more than 30 passages following the cell culture manual.
Green Fluorescent NIH/3T3 Cell Line (aka LINTERNA NIH/3T3) has been developed through stable transfection of NIH/3T3 cell line with tGFP. This cell line expresses green fluorescent protein gene sequences as free cytoplasmatic proteins. Each vial of cells contains more than 3 million viable cells stably expressing tGFP with puromycin resistance gene:
LINTERNA NIH/3T3 Cell line is ready to use in cell-based assay applications. This stably transfected clonal cell line provides consistent levels of expression, which helps simplify the interpretation of results. Green Fluorescent NIH/3T3 Cell Line also allows to stablish in vitro models for HIgh Throughput and High Content Screening.
Green Fluorescent Immortalized Human Microglia 
