INNOPROT has developed a proprietary drug discovery technology platform for multiplexing GPCR screening campaings using different fluorescent biosensors. This technology called Nomad Biosensors allows the measurement in vitro of the GPCRs different activation pathways performing one single assay.

Amongst NOMAD’s many advantages, you can easily evaluate G protein and β-arrestin modulation in the same assay, hence allowing biased activity studies. Four NOMAD biosensors are available for detection of Ca2+, cAMP, DAG or β-arrestin recruitment. Any combination of NOMAD biosensors can be co-expressed with the target tag-free GPCR, according to screening objectives.

 

Key Features:

  • G-protein and β-arrestin signaling modulation can be measured in the same assay
  • No need to tag the target GPCR
  • Direct measurement of Ca2+ ,cAMP or DAG flux ,and β-arrestin recruitment
  • Assay have high Z’ scores and low background
  • Adapted to HTS (384 well plate format)
  • Compound activity can be measured by fluorescence intensity or sensor translocation
  • Low running costs

Innoprot offers over 50 off-the-shelf NOMAD cell lines, and can also custom develop NOMAD cell lines as a service. NOMAD cell lines can be transferred to the client or used in contract research projects at Innoprot. Time to delivery of a new assay in 384 well plate-adapted format is typically about 2-4 months. Please contact us for more information –we would be pleased to discuss how NOMAD may help accelerate your research program.

More information

Contact us to learn more about NOMAD technology and how to boost your drug screening campaigns with automated imaging techniques using our biosensors.

Nomad Biosensors for Multiplexing GPCR Screening

Evaluation Kits:

Nomad® Biosensor Evaluation Kits allow use of the technology during six months evaluation period. There are four different kits depending on the different versions of Nomad® Biosensors. They provide all the necessary tools for evaluating the technology with the users’ own instrumentation. This offer allows users to become comfortable with the technology, before proceeding to a tiered licensing option that meets users’ specific needs.

Ref: P70752 – ENDRB Nomad Mpx Cell Line – Components

  • Stable Cell Line expressing ENDRB receptor & both Ca++ & cAMP biosensors
  • Demo Plate (Imaging grade plate with fixed cells to assist with instrument validation and image acquisition and analysis)
  • Two months testing purposes only limited Label License
  • Nomad Technology User Guide (complete with protocols)

Ref: P70746 – NTSR1 Nomad® Mpx Cell Line – Components

  • Stable Cell Line expressing NTSR1 receptor & both Ca++ & b-arrestin biosensors
  • Demo Plate (Imaging grade plate with fixed cells to assist with instrument validation and image acquisition and analysis)
  • Two months testing purposes only limited Label License
  • Nomad Technology User Guide (complete with protocols)

Ref: P70603 – M5 DAG-Nomad® Cell Line- Components

  • Stable Cell Line expressing M5 receptor & DAG biosensor
  • Demo Plate (Imaging grade plate with fixed cells to assist with instrument validation and image acquisition and analysis)
  • Two months testing purposes only limited Label License
  • Nomad Technology User Guide (complete with protocols)

Please contact us for detailed information regarding these licenses.