Innoprot offers a number of high throughput and high content assays for GPCR screening analyzing the different signalling pathways associated to GPCR activation. These assays use the Nomad Biosensors Technology, which allows to perform biased activity studies monitoring both G-Protein and β-arrestin pathways in living cells.

We can perform different assays with the target GPCR, according to your screening objectives:

  • cAMP
  • Calcium
  • Diacylglycerol
  • β-arrestin
  • Receptor Internalization

GLP-1 Receptor

GLP-1 Glucagon Receptor Assay

The GLP-1 Glucagon Receptor; a.k.a. GLP1R; is a receptor protein which is present in beta cells of the pancreas and also on neurons of the brain. GLP-1 Glucagon Receptor Assay from Innoprot allows the measurement of GLP1R receptor activation using Nomad biosensors technology. GLP-1 Glucagon Receptor signaling occurs via adenylate cyclase stimulation through trimeric Gs…

FPR2-ALX receptor internalization Assay

FPR2/ALX Formylpeptide Receptor Internalization Assay

FPR2/ALX Formylpeptide Receptor Internalization Assay from Innoprot allows to assay compounds, or analyze their capability to modulate FPR2/ALX receptor activation and the following redistribution process inside the cells. When a ligand binds to the FPR2/ALX, it activates a G protein, which internalizes in big and high intensity vesicles. This assay has been validated measuring Fluorescent…

EDNRA

ETA Endothelin Receptor Assay

ETA Endothelin Receptor Assay from Innoprot allows to assay compounds, or analyze their capability to modulate EDNRA. When a ligand binds to the EDNRA, it activates a G protein, which in turn, triggers a cellular response comprising Calcium. Our EDNRA assay measures Calcium concentration changes in the cytosol using Endothelin I as positive control. The…

ETB Endothelin Receptor Assay

ETB Endothelin Receptor Assay

ETB Endothelin Receptor Assay from Innoprot allows to assay compounds, or analyze their capability to modulate EDNRB. When a ligand binds to the EDNRB, it activates a G protein, which in turn, triggers a cellular response comprising both Calcium and cAMP. Our EDNRB assay measures both, cAMP & Calcium concentration changes in the cytosol using…

D5 Dopamine Receptor Cell Line

D5 Dopamine Receptor Assay

D5 Dopamine Receptor Assay from Innoprot allows the measurement of DRD5 receptor activation by cAMP increase in cell cytosol. We assay test-compounds analyzing the G-Protein signalling pathway by Gs involving DRD5 receptor activation. When an agonist binds to DRD5, it activates the Gs protein which; in turn, triggers a cellular response mediated by cAMP via…

D3 Dopamine Receptor Internalization Assay

D3 Dopamine Receptor Internalization Assay

D3 Dopamine Receptor Internalization Assay from Innoprot allows to assay compounds, or analyze their capability to modulate DRD3 receptor activation and the following redistribution process inside the cells. When a ligand binds to the DRD3, it activates a G protein, which internalizes in big and high intensity vesicles. This assay has been validated measuring Fluorescent…

D2 Dopamine Receptor Cell Line

D2 Dopamine Receptor Assay

D2 Dopamine Receptor Assay from Innoprot allows the measurement of DRD2 receptor activation by Adenylate cyclase inhibition. We assay test-compounds analyzing the G-Protein signalling pathway by Gi/o activation, which involves DRD2 receptor activation. When an agonist binds to DRD2, it activates the Gi/o protein which; in turn, triggers a cellular response mediated by cAMP via…

D1 Dopamine Receptor Cell Line

D1 Dopamine Receptor Assay

D1 Dopamine Receptor Assay from Innoprot allows the measurement of DRD1 receptor activation by cAMP increase in cell cytosol. We assay test-compounds analyzing the G-Protein signalling pathway by Gs involving DRD1 receptor activation. When an agonist binds to DRD1, it activates the Gs protein which; in turn, triggers a cellular response mediated by cAMP via…

CRF2 assay

CRF2 Corticotropin-releasing factor receptor assay

CRF2 Corticotropin-releasing factor receptor assay from Innoprot allows to assay compounds, or analyze their capability to modulate CRF2. When a ligand binds to the CRF2, it activates a G protein, which in turn, triggers a cellular response mediated by cAMP and calcium. CRHR2 assay comprises the measurment of both cAMP and calcium concentration changes in…