Innoprot assay development platform combinens both; target-based and phenotypic screening due to their important role to move from in-vitro to in-vivo pharmacology for a high range of disease pathways. It uses the best detection technology for a given molecular target and screening objective.

Innoprot offers in vitro models for many diseases; including Alzheimer’s DiseaseParkinson’s DiseaseAmyotrophic Laterial Sclerosis or Ischemic Stroke. These models mimick known disease pathways and then, depending on the disease model, analyze one specific endpoint or a wide variety of morphological analysis; such as neurite outgrowth, nuclear damage, membrane damage, oxidative stress, mitochondrial damage or functional activity.

Astrocytosis Assay

Astrocytosis Assay

Our Astrocytosis assay enables the study of fibrosis-related parameters on TGF-β1 induced Human primary astrocytes. Astrocytosis assay from Innoprot  allows the evaluation of therapeutic compounds in this disease area by measuring several biomarkers; including Fibronectin, ASMA and also Collagen via high content analysis. Astrocytosis is a common neurocellular manifestation of brain pathology in individuals with…

Cardiac Fibrosis Assay

Cardiac Fibrosis Assay

Cardiac Fibrosis Assay from Innoprot enables monitoring TGF-β/Smad signaling activation on Human Cardiac Fibroblasts. Immortalized primary Human Cardiac Fibroblasts stimulated with TGF-β1 demonstrate a clear concentration-dependent increase in Smad Binding Elements (SBE) expression; while increasing the TGF-β1 stimuli showes no effect of on the number of nuclei, indicative of no cytotoxic events. TGF-β trigger is…

Renal Fibrosis Assay

Renal Fibrosis Assay

Renal Fibrosis Assay from Innoprot enables the study of fibrosis-related parameters on TGF-β induced Renal Tubular Epithelial Cells. Human Renal Tubular Epithelial Cells stimulated with TGF-β1 demonstrate a clear concentration-dependent increase in αSMA levels; while increasing the TGF-β1 stimuli showes no effect of on the number of nuclei, indicative of no cytotoxic events. TGF-β trigger…

Endothelial Cell Tube Formation Assay

Endothelial Cell Tube Formation Assay

Endothelial Cell Tube Formation Assay from Innoprot allows to analyze the effect of test-compounds to induce/inhibit new blood vessels formation. We determine the IC50 values of your compounds of interest in whole cell assays using different organ-specific endothelial cells; such as liver, renal, retinal, colonic, lung, pancreas or umbilical endothelial cells. We offer over 20…

Tumor Cell Proliferation Assay

Tumor Cell Proliferation Assay

Tumor Cell Proliferation Assay from Innoprot includes allows to analyze the effect of test-compounds to increase/decrease cell proliferation. We determine the IC50 values of your compounds of interest in whole cell assays using different techniques; such as MTT, LDH release, CellTiter or nuclei quantification. We offer over 60 different tumor cell lines for cell screening…

Aβ1-40 Induced Neurotoxicity Assay

Aβ1-40 Induced Neurotoxicity Assay

Intoxication of neurons with beta-amyloid (1-40) is a useful Alzheimer disease (AD) in vitro model. This model allows evaluate the capacity of different agents to inhibit the neuronal damage in AD based on the accumulation of neurotoxic amyloid beta peptide. Aβ1-40 Induced Neurotoxicity Assay offers the advantage of a rapid screening of the neuroprotective potential…

Aβ1-42 Induced Neurotoxicity Assay

Aβ1-42 Induced Neurotoxicity Assay

Intoxication of neurons with beta-amyloid (1-42) oligomers is a useful Alzheimer disease (AD) in vitro model. This model allows evaluate the capacity of different agents to inhibit the neuronal damage in AD based on the accumulation of neurotoxic amyloid beta oligomers. Aβ1-42 Induced Neurotoxicity Assay offers the advantage of a rapid screening of the neuroprotective…

In vitro Cell Migration Assay

In vitro Cell Migration Assay

Tumor cell migration/invasion is one of the key steps in the tumor progression. The metastatic potential of tumor cells is largely dependent on their ability to degradate and migrate through extracellular matrix. In vitro cell migration assay from Innoprot is a Boyden Chamber assay. Boyden Chambers consist of a cell culture insert nested in the…

In vitro Cell Invasion Assay

In vitro Cell Invasion Assay

Tumor cell migration/invasion is one of the key steps in the tumor progression. The metastatic potential of tumor cells is largely dependent on their ability to degradate and migrate through extracellular matrix. In vitro cell invasion assay from Innoprot is a Boyden Chamber assay. Boyden Chambers consist of a cell culture insert nested in the…