Innoprot offers both target-based and phenotypic in vitro assays to identify new test-compounds against Alzheimer’s Disease.
Our phenotypic assays mimick specific disease conditions on primary neurons measuring their responses in combinantion with compounds incubation.
In another hand, our target-based assays mimmick specific pathways to monitor them using different reporter genes.
Intoxication of neurons with beta-amyloid (1-40) is a useful Alzheimer disease (AD) in vitro model. This model allows evaluate the capacity of different agents to inhibit the neuronal damage in AD based on the accumulation of neurotoxic amyloid beta peptide. Aβ1-40 Induced Neurotoxicity Assay offers the advantage of a rapid screening of the neuroprotective potential…
Intoxication of neurons with beta-amyloid (1-42) oligomers is a useful Alzheimer disease (AD) in vitro model. This model allows evaluate the capacity of different agents to inhibit the neuronal damage in AD based on the accumulation of neurotoxic amyloid beta oligomers. Aβ1-42 Induced Neurotoxicity Assay offers the advantage of a rapid screening of the neuroprotective…
Gamma secretase activity assay from Innoprot allows the monitoring of γ-secretase proteolytic process in living cells. Assay is performed on a cell line expressing a fluorescent construct (APP-C99) containing the γ-secretase cleaving site. When γ-secretase activity is inhibited by any antagonist, fluorescent aggregates appear in the cell cytoplasm in a dose-response manner.
tau phosphorylation assay from Innoprot allows the identification of tau kinase and phosphatase inhibitors and modulators that affect the behaviour or location of tau protein. Tau is a group of neuronal microtubule-associated proteins that are formed by alternative mRNA splicing. They accumulate in neurofibrillary tangles in Alzheimer’s disease (AD) brain. There is significant evidence that…