Effective cell-based assays for predicting organ-specific toxicity potential are needed early in drug dicovery for screening and characterization of toxicity. The High Content Screening platform of Innoprot allows to investigate the cellular toxicity on hundreds of drug candidates using the most efficient technology, analyzing all the different events that a toxicant could induce in a specific cell type.

In our organ-specific toxicity assays, we analyze different parameters involved with toxicity,in a single assay including general paramenters like oxidative stress, apoptosis, mitochondrial damage or more specific parameters like neurite outgrowth related with neurotoxicity.

DNA Damage Assay

DNA Damage Assay

Several processes or insults can induce damage on DNA; such as a cell death pathway activation or by the genotoxic activity of a compound; substances that makes them harmful to the genetic information contained in organisms. Genotoxins are the substances that have the property of genotoxicity. All new chemical entities in the drug discovery process…

Mitochondrial Integrity Assay

Mitochondria are cellular organelles regulating metabolism and cell death pathways. The membrane of the mitochondria is the key component to maintain the cell metabolism and homeostasis. Loss of membrane potential of mitochondria is the previous event of the cell dysfunction; a pathway directly related with the activation of death pathways via caspase 3 activation. Mitochondrial Integrity…

Oxidative Stress Assay

The oxidative stress is an imbalance between the production of reactive oxygen strains (ROS); and the capacity of the cell to remove them. The excess of these strains finally produce peroxides and free radicals that damage cell components and compromise the cell functions. Oxidative Stress Assay from Innoprot uses a specific probe (CM-H2DCFDA) to evaluate…

Nephrotoxicity Assay

Nephrotoxicity Assay

Proximal tubules of the kidneys are one of the most common targets of nephrotoxic drugs and chemicals. Screens to predict nephrotoxic potential of compounds with insights to mechanisms of toxicity facilitate lead optimization, guide structure-activity relationships, minimize risks of clinical nephrotoxicity and therefore are valuable in the process of drug discovery. Innoprot has developed an…

Cardiotoxicity Assay

Cardiotoxicity Assay

Cardiotoxicity is a condition when the heart has problems to pump properly. One of the most common events for a heart dysfunction is the toxicity of the functional cardiac cells –cardiomyocytes-. The most usual mechanisms involved in the cardiac cell dysfunction are; the oxidative extress, the DNA damage and the caspase activation with the subsequent…

Neurotoxicity Assay

Neurotoxicity Assay

Neurotoxicity is the capacity of certain compounds to affect the normal activity of the neurons. The mechanism involving the neuron citotoxicity can be several: oxidative stress, DNA damage, cytoskeleton dysfunction or apoptosis. We analyze the following parameters in the Neurotoxicity Assay: Cell Viability Apoptosis: Caspase 3/7 Activation Membrane Damage Neurite Outgrowth Mitochondrial Damage In this…

Hepatotoxicity Assay

Hepatotoxicity Assay

Drug-Induced Liver Injury (DILI) is the most common event causing during the drug withdrawals. But the causes of the hepatic failure provoked by the chemical compounds could be several. Due to this reason, a multiparametric assay is needed to predict toxicities that could be hidden in a simple one parameter assay. We analyze the following…

Neurite Outgrowth Assay

Neurite Outgrowth Assay

Neurite outgrowth is a key process during neurogenesis. During development, neurons extend numerous processes that differentiate into dendrites and axons. These processes, also termed neurites, are critical for communication between neurons. The initiation of neurite protrusion and subsequent elongation involves complex intracellular signaling. Neurites are particularly interesting in relation to neuropathological disorders, neuronal injury/regeneration, and…