About 10% of ALS cases are inherited; and a large subset of them are caused by mutations in the gene encoding the copper-zinc superoxide dismutase (SOD1). The detection of SOD1-positive inclusions in familial ALS patients suggests the role of SOD1 aggregation underlying the pathology of familial ALS. Although SOD1 mutant proteins are different in structure,…
α-Synuclein is a presynaptic neuronal protein which may contribute genetically and neuropathologically to Parkinson’s disease (PD). Aggregated a-synuclein is the major component of inclusions in Parkinson’s disease; but also in other synucleinopathy brains indicating that α-synuclein aggregation may play a main role in the pathogenesis of neurodegenerative disorders. In this α-synuclein aggregation assay from Innoprot,…
alpha1A Adrenoceptor assay (ADRA1A) from Innoprot provides the analysis of both signalling pathways involving receptor activation; G protein by Calcium flux and β-arrestin recruitment using Nomad multiplex technology. The alpha-1A adrenergic receptor (α1A adrenoreceptor), also known as ADRA1A, is member of Alpha-1-adrenergic receptors (alpha-1-ARs) family. They activate mitogenic responses and regulate growth and proliferation of…
alpha1B Adrenoceptor assay (ADRA1B) from Innoprot provides the analysis of both signalling pathways involving receptor activation; G protein by Calcium flux and β-arrestin recruitment using Nomad multiplex technology. The alpha-1B adrenergic receptor (α1B adrenoceptor), also known as ADRA1B, is member of Alpha-1-adrenergic receptors (alpha-1-ARs) family. They activate mitogenic responses and regulate growth and proliferation of…
CCK2R (CCKBR) Human Cholecystokinin receptor assay from Innoprot provides the analysis of both signalling pathways involving GPCR activation; G protein by Calcium flux and β-arrestin recruitment using Nomad multiplex technology. The cholecystokinin B receptor (CCKBR, CCK2R) is a protein that in humans is encoded by the CCKBR gene. This gene encodes a G-protein-coupled receptor for…
Human Neurotensin receptor 1 assay (NTSR1) from Innoprot provides the analysis of both signalling pathways involving GPCR activation; G protein by Calcium flux and β-arrestin recruitment using Nomad multiplex technology. The primary transduction mechanism involves Gq/G11 family transducers, so we measure Ca2+ flux in combination with β-arrestin recruitment in this assay. Neurotensin receptor 1 belongs to…
Ischemic Stroke in vitro assay is based on the exposure of rat primary neurons under Oxygen-Glucose Deprivation conditions. Ischemia-like conditions are induced by replacing the normal O2/CO2 equilibrated medium with 94,7% N2 /5% CO2/0,3% O2 medium in a hypoxic chamber. We also use a culture medium without glucose to expose the cells to real OGD…
Amyloid-beta peptide Oligomerization Assay is a functional cell-based assay to identify inhibitors of amyloid peptide oligomerization process. We incubate rat primary neurons, human Microglia or SH-SY5Y cells with test compounds in parallel with Aβ1-42 peptide during 24h. After that, we measure Aβ1-42 oligomerization process analysing the number of thiazine staining oligomers related to cell number .
Alzheimer’s Disease (AD) comprises the accumulation of brain depositions of the beta amyloid (βA). The βA is the amyloid precursor protein (APP) digestion product, released from the cell after secretases proteolysis.This is the basis of the APP Processing Assay offered by Innoprot. Innoprot has developed a novel fluorescence-based assay for screening of inhibitors of the…