Renal Fibrosis Assay from Innoprot enables the study of fibrosis-related parameters on TGF-β induced Kidney Fibroblasts and/or Kidney Mesangial Cells.
Immortalized human primary renal mesangial cells and/or fibroblasts stimulated with TGF-β1 demonstrate a clear concentration-dependent increase in αSMA levels; while increasing the TGF-β1 stimuli showes no effect of on the number of nuclei, indicative of no cytotoxic events. TGF-β trigger is inhibited by treatment with the ALK-5 inhibitor, SB525334, showing full inhibition of αSMA regardless of the presence of TGF-β1. Two different ways to measure changes are performed, actin stained area and fiber length in this Renal Fibrosis Assay.
