_CaNOMAD Muscarinic Acetylcholine Receptor M1 Cell Line

_CaNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and Calcium Nomad Biosensor to monitor G-protein modulation by intracellular calcium release. Each vial of _CaNOMAD CHRM1 Receptor Cell Line contains U2OS cells stably expressing the following constructs:

• human Muscarinic Receptor 1 (CHRM1) with no tag
• Calcium Nomad Biosensor (Green Fluorescence)

_CaNOMAD Muscarinic Acetylcholine Receptor M1 Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gq/11 involving receptor activation. When an agonist binds to M1 receptor, the Gq/G11 protein is activated which, in turn, triggers a cellular response mediated by calcium via Phospholipase C stimulation. This leads to an increase of green fluorescence intensity of Calcium biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.