Fluorescent LMNB1 Cell Line

Reference: P30601

Fluorescent LMNB1 Cell Line

Novel Red Fluorescent LMNB1 Cell Lines have been developed through stable transfection with LMNB1 endogenously tagged with TagRFP in U2OS & SH-SY5Y cell lines. Both cell lines allow the monitorization of cellular apoptosis level through nuclear lamin B1 morphological changes in cell-based assays.

Each vial of both U2OS/LMNB1-TagRFP and SH-SY5Y/LMNB1-TagRFP cell lines contains 3 million cells. It is guaranteed that these cells will expand following the cell culture manual.


Size/Quantity: 1 vial containing 3×106 cells / vial


The lamin are type V intermediate filaments which provides structure and mechanical support to the cell nucleus. Besides, they play important roles in nuclear stability, reassembly of the nuclear envelope during mitosis, chromatin structure, anchoring of nuclear pore complexes and gene expression. Lamin B1 (LMNB1) is essential for proper chromosome condensation in interphase nuclei. It also participates in the distribution of heterochromatin and modulation of gene expression and splicing. It plays a key role in DNA replication, cellular aging and stress responses. Innoprot fluorescent LMNB1 Cell Line allows to assay compounds for their ability to induce apoptosis analyzing their nuclear stability.

To validate the fluorescent LMNB1 Cell Line as apoptosis model, we incubate the cells with staurosporine. In non-apoptotic cells TagRFP appears localized in nuclear envelope but after treatment, activated caspase family proteases cleave lamin, resulting in the loss of its structure and detachment from chromatine. Tagged lamin appears disassembled and forms round aggregates. The assay was developed and optimized using the BD Pathway HCS Reader and Attovision Compartimentalization Software. The parameters analyzed in order to check the apoptosis degree are eccentricity and perimeter of the nucleus.



  • Reference: P30601
  • Antibiotic Resistance: G418
  • Size/Quantity: 1 vial containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice