Green Fluorescent Immortalized HBMEC

Reference: P10361-IM-tGFP

Green Fluorescent Immortalized HBMEC

Green Fluorescent Immortalized Human Brain Microvascular Endothelial Cells (IM-HBMEC-tGFP) provided by Innoprot have been developed by immortalizing primary human brain endothelial cells with SV40 Large T antigen and transfected with tGFP.

Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 3rd passage while the SV40‐tranduced cells go beyond 30 passages.

Each vial of IM-HBMEC-tGFP contains more than 1 million viable cells. Innoprot also offers optimized medium and reagents for the growth of IM-HBMEC-tGFP which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro IM-HBMEC-tGFP culture.


Size/Quantity: 1 million cells / vial


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Human Brain microvascular endothelial cells (BMEC) is the major component of the blood-brain barrier. One of their functions is to limit the passage of soluble and cellular substances from the blood into the brain. BMEC have unique features to distinguish themselves from those of peripheral endothelial cells, such as; 1) intercellular tight junctions that display high electrical resistance and also a slow paracellular flux; 2) the absence of fenestrae and a reduced level of pinocytic activity; and 3) asymmetrically-localized enzymes and carrier-mediated transport systems. Similar to peripheral endothelial cells, BMEC can express cell adhesion molecules on their surface that regulate the extravasation of leukocytes into the brain. BMEC are useful tools for studying the molecular and cellular properties of blood-brain barrier because of their unique functions. Immortalized Human Brain Microvascular Endothelial Cells allow also to stablish in vitro models for HIgh Throughput Screening and High Content Screening.


About turboGFP protein:

tGFP is an improved variant of the green fluorescent protein CopGFP from copepoda Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins. TurboGFP is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.

  • Reference: P10361-IM-tGFP
  • Size/Quantity: 1x106 cells / vial
  • Fluorescent Protein: turboGFP (excitation/emission max = 482/502 nm respectively)
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice

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