Green Fluorescent MDCK Cell Line

Reference: P20106

950,00

Green Fluorescent MDCK Cell Line (aka LINTERNA MDCK) has been developed through stable transfection of MDCK cell line with tGFP. This cell line expresses green fluorescent protein gene sequences as free cytoplasmatic proteins. Each vial of cells contains more than 3 million viable cells stably expressing tGFP with G418 resistance gene:

LINTERNA MDCK Cell line is ready to use in cell-based assay applications. This stably transfected clonal cell line provides consistent levels of expression, which helps simplify the interpretation of results. Green Fluorescent MDCK Cell Line also allow to stablish in vitro models for HIgh Throughput and High Content Screening.

Green Fluorescent MDCK Cell Line are suitable for both, in vitro and in vivo experimentation. About MDCK Cell line: MDCK cell line comes from a kidney of an apparently normal adult female cocker spaniel. They are epithelial-like cells growing adherent as monolayer and also in cell clusters. The cells are positive for keratin by immunoperoxidase staining. MDCK cells have been used to study processing of beta amyloid precursor protein and sorting of its proteolytic products. They are also useful in viral studies, supports the growth of vesicular exanthema virus of swine, vesicular stomatitis, vaccina, coxsackie B-5, adenoviruses, reoviruses and infectious canine hepatitis. About turboGFP protein: tGFP is an improved variant of the green fluorescent protein CopGFP from copepoda Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins. TurboGFP is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.
  • Reference: P20106
  • Size/Quantity: 3x10⁶ cells / vial
  • Resistance Gene: G418
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice

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