Green Fluorescent NIH/3T3 Cell Line

Reference: P20105

Green Fluorescent NIH/3T3 Cell Line

Green Fluorescent NIH/3T3 Cell Line (aka LINTERNA NIH/3T3) has been developed through stable transfection of NIH/3T3 cell line with tGFP. This cell line expresses green fluorescent protein gene sequences as free cytoplasmatic proteins. Each vial of cells contains more than 3 million viable cells stably expressing tGFP with puromycin resistance gene:

LINTERNA NIH/3T3 Cell line is ready to use in cell-based assay applications. This stably transfected clonal cell line provides consistent levels of expression, which helps simplify the interpretation of results. Green Fluorescent NIH/3T3 Cell Line also allows to stablish in vitro models for HIgh Throughput and High Content Screening.


Green Fluorescent NIH/3T3 Cell Line are suitable for both, in vitro and in vivo experimentation.

About NIH/3T3 Cell line:

NIH 3T3 mouse embryonic fibroblast cells come from a cell line isolated in 1962. 3T3 refers to the cell transfer and inoculation protocol for the line, and means “3-day transfer, inoculum 3 x 105 cells”. Using this protocol, the immortal cell line begins to thrive and stabilize in cell culture after about 20-30 generations of in vitro growth. The scientists who first cultured this cell line, obtained the cells from desegregated NIH Swiss mouse embryo fibroblasts. The cell line has since become a standard fibroblast cell line. The original cells are extremely contact inhibited, although the cell line is no longer inhibited. 3T3 cells are sensitive to sarcoma virus focus formation, as well as leukemia virus.

About turboGFP protein:

tGFP is an improved variant of the green fluorescent protein CopGFP from copepoda Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins. TurboGFP is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.

  • Reference: P20105
  • Size/Quantity: 3x106 cells / vial
  • Resistance Gene: Puromycin
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice