Immortalized Human Epidermal Keratinocytes

Reference: P10852-IM

2.085,00€

Immortalized Human Epidermal Keratinocytes (IM-HEK) provided by Innoprot have been developed by immortalizing primary human epidermal keratinocytes with SV40 Large T antigen.

Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 5th passage while the SV40‐tranduced cells go beyond 30 passages.

Each vial of Immortalized Human Epidermal Keratinocytes contains more than 1 million viable cells. Innoprot also offers optimized medium and reagents for the growth of IM-HEK which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro IM-HEK culture.

The epidermal layer of the skin provides an essential function as a protective barrier against insults from the external environment. The predominant cell type in the epidermis is keratinocytes and they are present in the stratified squamous epithelia. Progenitors of keratinocytes reside and divide in the basal layer of the epidermis. They then differentiate, migrate towards the surface of epidermis, and eventually withdraw from the cell cycle permanently. Keratinocyte proliferation, differentiation, and programmed cell death are complex and carefully choreographed processes. Apart from their protective functions, Human Epidermal Keratinocytes express adhesion molecules and cytokines, further suggesting an implication in skin innate immunity, tissue homeostasis, wound healing, cancer development, and skin-based gene-therapy. Immortalized Human Epidermal Keratinocytes are also useful tools to stablish in vitro disease models for High Throughput and High Content Screening.