MPXNOMAD CB1 Cannabinoid Receptor Cell Line

Reference: P70727

MPXNOMAD CB1 Cannabinoid Receptor Cell Line

MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD CB1 Cannabinoid Receptor Cell Line contains HEK293 cells stably expressing the following constructs:

  • human α1B-adrenoceptor with no tag
  • cAMP Nomad Biosensor (Red Fluorescence)
  • β-arrestin Nomad Biosensor (Green Fluorescence)

MPXNOMAD CB1 Cannabinoid Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by cAMP flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to CB1 a G protein is activated which, in turn, triggers a cellular response mediated by cAMP and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and cAMP biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.


Size/Quantity: 2 vials containing 3×106 cells / vial


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The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two cannabinoid receptors play and important role in the cannabinoid-induced CNS effects, such as alterations in mood and cognition. CB1 receptors appear in the CNS and are responsible for mediating the effects of cannabinoid binding in the brain. Depolarized neurons release endocannabinoids, which bind to CB1 receptors on pre-synaptic glutamatergic and GABAergic neurons, resulting in a respective decrease in either glutamate or GABA release. MPXNOMAD CB1 Cannabinoid Receptor Cell Line from Innoprot allows the measurement of the activity and potency of ligands; such as CP55940.

Click HERE to know more about CB1 Cannabinoid Receptor.


  • Reference: P70727
  • Nomad Biosensors: β-arrestin & cAMP
  • NCBI Reference Sequence: AY225225.1
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice