MPXNOMAD CCK2 Cholecystokinin Receptor Cell Line

Reference: P70708

MPXNOMAD CCK2 Cholecystokinin Receptor Cell Line

MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD CCK2 Cholecystokinin Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human CCK2 Cholecystokinin Receptor with no tag
  • Calcium Nomad Biosensor (Green Fluorescence)
  • β-arrestin Nomad Biosensor (Red Fluorescence)

MPXNOMAD CCK2 Cholecystokinin Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to CCK2R a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.


Size/Quantity: 2 vials containing 3×106 cells / vial


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CCK receptors significantly influence neurotransmission in the brain, regulating anxiety, feeding, and locomotion. Two GPCRs known as CCK1 and CCK2 receptors composes the CCK receptors family. CCK2R gene encodes a G protein-coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. Cholecystokinin B receptor is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is present principally in the central nervous system and the gastrointestinal tract. CCK-B expression may correlate parallel to anxiety and depression phenotypes in humans. MPXNOMAD CCK2 Cholecystokinin Receptor Cell Line from Innoprot allows the measurement of the activity and potency of ligands; such as CCK.

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  • Reference: P70708
  • Nomad Biosensors: β-arrestin & Ca++
  • Gene Bank Acc. Nº: AY322551
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice