MPXNOMAD H1 Histamine Receptor Cell Line

Reference: P70735

MPXNOMAD H1 Histamine Receptor Cell Line

MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD H1 Histamine Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human H1 Histamine Receptor with no tag
  • Calcium Nomad Biosensor (Green Fluorescence)
  • β-arrestin Nomad Biosensor (Red Fluorescence)

MPXNOMAD H1 Histamine Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to H1 receptor a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.


Size/Quantity: 2 vials containing 3×106 cells / vial


The H1 receptor is a histamine receptor belonging to the family of rhodopsin-like G-protein-coupled receptors. Histamine H1 receptor is present in several tissues such as central nervous system, cardiovascular system, smooth muscles, or lymphocytes. In vascular endothelial cells, stimulation of histamine H1 receptors lead to the synthesis and release of several neurochemicals and neuromodulators; such as prostacyclin, platelet–activating factor, and nitric oxide (NO). In addition, stimulation of the H1 receptor causes changes in vascular permeability, particularly the postcapillary venule as a result of endothelial cell contraction. Antihistamines, which act on this receptor, are anti-allergy drugs. MPXNOMAD H1 Histamine Receptor Cell Line from Innoprot allows the measurement of the activity and potency of ligands; such as Histamine.

Click HERE to know more about H1 Receptor.


  • Reference: P70735
  • Nomad Biosensors: β-arrestin & Ca++
  • Gene Bank Acc. Nº: AY136743
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice