MPXNOMAD PAR2 Receptor Cell Line

Reference: P70723

MPXNOMAD PAR2 Receptor Cell Line

MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXNOMAD PAR2 Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human PAR2 Receptor with no tag
  • Calcium Nomad Biosensor (Green Fluorescence)
  • β-arrestin Nomad Biosensor (Red Fluorescence)

MPXNOMAD PAR2 Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to PAR2 Receptor a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.


Size/Quantity: 2 vials containing 3×106 cells / vial


Proteinase-activated receptor 2 (PAR2) also known as coagulation factor II (thrombin) receptor-like 1 (F2RL1) or G-protein coupled receptor 11 (GPR11) is a protein that in humans is encoded by the F2RL1 gene. PAR2 modulates inflammatory responses, obesity, metabolism, and acts as a sensor for proteolytic enzymes generated during infection. Functional selectivity occurs with PAR2, several proteases cleave PAR2 at distinct sites leading to biased signalling. Synthetic small ligands also modulate biased signalling leading to different functional responses. So far, PAR2 has been co-crystallized with two different antagonist ligands, while an agonist-bound state model of PAR2 (with the endogenous ligand SLIGKV) has been determined through mutagenesis and structure-based drug design. MPXNOMAD PAR2 Receptor Cell Line from Innoprot allows the measurement of the activity and potency of ligands; such as trypsin.

Click HERE to know more about PAR2 Receptor.


  • Reference: P70723
  • Nomad Biosensors: β-arrestin & Ca++
  • Gene Bank Acc. Nº: NM-005242.4
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice