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M4 Muscarinic Acetylcholine Receptor Assay

Muscarinic Receptors

M4 Muscarinic Acetylcholine Receptor Assay (CHRM4) from Innoprot measures CHRM4 receptor activation using Nomad Biosensors technology. CHRM4 signaling occurs via adenylate cyclase inhibition through trimeric Gi/o proteins, reducing cAMP via PKA activation.

In this assay, we measure the receptor activation/inhibition by PKA activation using Nomad Biosensors technology. The high reproducibility of this assay allows monitoring M4 Muscarinic Acetylcholine Receptor activation process in living cells.

Receptor Information: M4 Muscarinic Acetylcholine Receptor

Cell line used:CHRM4 Nomad Cell Line

Readout: cAMP Flux (Gi/o activation)

Agonist: Oxotremorine

EC50 Agonist: 4.72 x 10^-8 M for Gi/Go Activation

Type of Assay: Cell-Based/Functional
Detection Method: Fluorimetry

Assay: Muscarinic Receptors

Type: Functional GPCR Screening Services

Cell Line Information

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