_MPXNOMAD EP1 Prostanoid Receptor Cell Line

_MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor both G-protein pathways. Each vial of _MPXNOMAD EP1 Prostanoid Receptor Cell Line contains U2OS cells stably expressing the following constructs:

• human EP1 Prostanoid Receptor with no tag
• Calcium Nomad Biosensor (Green Fluorescence)
• b-arrestin Nomad Biosensor (Red Fluorescence)

_MPXNOMAD EP1 Prostanoid Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; both G protein by Ca²⁺ flux and b-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to EP1 Prostanoid Receptor both signalling pathways are activated which, in turn, triggers a cellular response mediated by calcium and b-arrestin recruitment. This leads to an increase of fluorescence intensity of both b-arrestin and Ca²⁺ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×10⁶ cells / vial