<sub>cAMP</sub>NOMAD Adenosine A3 Receptor Cell Line - Imagen 2

<sub>cAMP</sub>NOMAD Adenosine A3 Receptor Cell Line - Imagen 3

_cAMPNOMAD Adenosine A3 Receptor Cell Line

Reference: P70524

9.995,00€

_cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of _cAMPNOMAD Adenosine A3 Receptor Cell Line contains HEK293/U2OS cells stably expressing the following constructs:

human Adenosine A3 receptor (ADORA3) with no tag
cAMP Nomad Biosensor (Red Fluorescence)

_cAMPNOMAD ADORA3 Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Go involving receptor activation. When an agonist binds to ADORA3, Go protein is activated which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×10⁶ cells / vial

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Limpiar

The adenosine A3 receptor (ADORA3) is one member of the adenosine receptor group of GPCRs with adenosine as endogenous ligand. Activation of the adenosine A3 receptor by an agonist causes binding of Gi1/2/3 or Go protein. Binding of Gi1/2/3 causes an inhibition of adenylate cyclase and, therefore, a decrease in the cAMP concentration. Adenosine A3 Receptor mediates a cardioprotective function during cardiac ischemia, it plays roles in the inhibition of neutrophil degranulation in neutrophil-mediated tissue injury, and also in both neuroprotective and neurodegenerative effects; and it may also mediate both cell proliferation and cell death Click HERE to know more about ADORA3 Receptor.