DAGNOMAD Muscarinic Acetylcholine Receptor M5 Cell Line

Reference: P70603

DAGNOMAD Muscarinic Acetylcholine Receptor M5 Cell Line

DAGNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and diacylglycerol (DAG) Nomad Biosensor to monitor G-protein modulation by DAG release. Each vial of DAGNOMAD Muscarinic M5 Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human Muscarinic M5 Receptor (M5R) with no tag
  • DAG Nomad Biosensor (Red Fluorescence)

cAMPNOMAD Muscarinic M5 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gq/G11 involving receptor activation. When an agonist binds to M5 Receptor, the Gq/G11 protein is activated which, in turn, triggers a cellular response mediated by DAG via Phospholipase C stimulation. This leads to an increase of red fluorescence intensity of DAG biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×106 cells / vial


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The muscarinic acetylcholine receptor family consists of five GPCRs that mediate some of the neurotransmission functions of acetylcholine in the CNS and the periphery. Muscarinic Acetylcholine Receptor M5 has a discrete expression profile appearing predominantly on dopamine neuron terminals; within the dorsal and ventral striatum that potentiate the release of dopamine and glutamate from midbrain projections. It is the sole mAChR in dopaminergic neurons of the ventral tegmental area and substantia nigra pars compacta. Furthermore, the M5 mAChR appears densely in the ventral subiculum; which projects to the NAc shell, an input pathway implicated in context-mediated relapse to ethanol seeking. DAGNOMAD Muscarinic Acetylcholine Receptor M5 Cell Line from Innoprot allows the measurement of the activity and potency of ligands; such as oxotremorine.

Click HERE to know more about Muscarinic 5 Receptor.

  • Reference: P70603
  • Nomad Biosensors: cAMP
  • GeneBank Accesion Number: M80333
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice