Green Fluorescent LS174T Cell Line

Reference: P20124

Green Fluorescent LS174T Cell Line

Green Fluorescent LS174T Cell Line (aka LINTERNA LS174T) has been developed through stable transfection of LS174T cell line with tGFP. This cell line expresses green fluorescent protein gene sequences as free cytoplasmatic proteins. Each vial of cells contains more than 3 million viable cells stably expressing tGFP with G418 resistance gene:

LINTERNA LS174T Cell line is ready to use in cell-based assay applications. This stably transfected clonal cell line provides consistent levels of expression, which helps simplify the interpretation of results. Green Fluorescent LS174T Cell Line also allow to stablish in vitro models for HIgh Throughput and High Content Screening.


Green Fluorescent LS174T Cell Line are suitable for both, in vitro and in vivo experimentation.

About LS174T Cell line:

LS174T melanoma cell line comes from a Duke’s type B adenocarcinoma of the colon of a 58 year-old Caucasian female patient. LS 174T is a variant of LS 180 which has been in culture by using trypsin in the subculture protocol. It is more easily subcultivated than that parent line and, like LS 180, it is reported to produce large amounts of carcinoembryonic antigen (CEA). Electron microscopic studies revealed abundant microvilli and intracytoplasmic mucin vacuoles. They are negative for p53 antigen expression, but positive for mRNA expression.

About turboGFP protein:

tGFP is an improved variant of the green fluorescent protein CopGFP from copepoda Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins. TurboGFP is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.

  • Reference: P20124
  • Size/Quantity: 3x106 cells / vial
  • Resistance Gene: G418
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice