PKANOMAD Muscarinic Acetylcholine Receptor M4 Cell Line

Reference: P70545

PKANOMAD Muscarinic Acetylcholine Receptor M4 Cell Line

PKANOMAD Cells are cell lines stably co-expressing tag-free GPCRs and PKA Nomad Biosensor to monitor G-protein modulation by PKA activation. Each vial of PKANOMAD Muscarinic M4 Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human Muscarinic M4 Receptor (M4R) with no tag
  • PKA Nomad Biosensor (Red Fluorescence)

PKANOMAD Muscarinic M4 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gi/Go involving receptor activation. When an agonist binds to M4 Receptor, the Gi/Go protein is activated which, in turn, triggers a cellular response mediated by cAMP via PKA activation. This leads to an increase of red fluorescence intensity of PKA biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×106 cells / vial


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The muscarinic acetylcholine receptor family consists of five GPCRs that mediate some of the neurotransmission functions of acetylcholine in the CNS and the periphery. Muscarinic 4 Receptor functions as inhibitory autoreceptor for acetylcholine. Activation of M4 receptors inhibits acetylcholine release in the striatum. The M2 subtype of acetylcholine receptor functions similarly as an inhibitory autoreceptor to acetylcholine release; albeit functioning actively primarily in the hippocampus and cerebral cortex. Muscarinic acetylcholine receptors possess a regulatory effect on dopaminergic neurotransmission. Activation of M4 receptors in the striatum inhibit D1-induced locomotor stimulation in mice. M4 receptor-deficient mice exhibit increased locomotor simulation in response to D1 agonists, amphetamine and cocaine. Neurotransmission in the striatum influences extrapyramidal motor control, thus alterations in M4 activity may contribute to conditions such as Parkinson’s disease. DAGNOMAD Muscarinic Acetylcholine Receptor M4 Cell Line from Innoprot allows the measurement of the activity and potency of ligands; such as oxotremorine.

Click HERE to know more about Muscarinic 4 Receptor.

  • Reference: P70545
  • Nomad Biosensors: cAMP
  • GeneBank Accesion Number: X15265
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice