_icAMPNOMAD Muscarinic Acetylcholine Receptor M4 Cell Line

Reference: P70545

9.995,00€

_icAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and PKA Nomad Biosensor to monitor G-protein modulation by icAMP activation. Each vial of _icAMPNOMAD Muscarinic M4 Receptor Cell Line contains HEK 293/U2OS cells stably expressing the following constructs:

Human Muscarinic M4 Receptor (M4R) with no tag
icAMP Nomad Biosensor (Red Fluorescence)

_icAMPNOMAD Muscarinic M4 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by G_i/G_o involving receptor activation. When an agonist binds to M4 Receptor, the G_i/G_o protein is activated which, in turn, triggers a cellular response mediated by icAMP. This leads to an increase of red fluorescence intensity of icAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×10⁶ cells / vial

Select cell line

Limpiar

The muscarinic acetylcholine receptor family consists of five GPCRs that mediate some of the neurotransmission functions of acetylcholine in the CNS and the periphery. Muscarinic 4 Receptor functions as inhibitory autoreceptor for acetylcholine. Activation of M4 receptors inhibits acetylcholine release in the striatum. The M2 subtype of acetylcholine receptor functions similarly as an inhibitory autoreceptor to acetylcholine release; albeit functioning actively primarily in the hippocampus and cerebral cortex. Muscarinic acetylcholine receptors possess a regulatory effect on dopaminergic neurotransmission. Activation of M4 receptors in the striatum inhibit D1-induced locomotor stimulation in mice. M4 receptor-deficient mice exhibit increased locomotor simulation in response to D1 agonists, amphetamine and cocaine. Neurotransmission in the striatum influences extrapyramidal motor control, thus alterations in M4 activity may contribute to conditions such as Parkinson’s disease. _icAMPNOMAD Muscarinic Acetylcholine Receptor M4 Cell Line from Innoprot allows the measurement of the activity and potency of ligands; such as oxotremorine. Click HERE to know more about Muscarinic 4 Receptor.