Red Fluorescent B16-F10 Cell Line

Reference: P20321

Red Fluorescent B16-F10 Cell Line

Red Fluorescent B16-F10 Cell Line (aka VAMPIRO B16-F10) has been developed through stable transfection of B16-F10 cell line with turboFP602. This cell line expresses red fluorescent protein gene sequences as free cytoplasmatic proteins. Each vial of cells contains more than 3 million viable cells stably expressing turboFP602 with Puromycin resistance gene:

VAMPIRO B16-F10 Cell line is ready to use in cell-based assay applications. This stably transfected clonal cell line provides consistent levels of expression, which helps simplify the interpretation of results. Red Fluorescent B16-F10 Cell Line also allow to stablish in vitro models for High Throughput and High Content Screening.


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Red Fluorescent B16-F10 Cell Line are suitable for both, in vitro and in vivo experimentation.


About B16-F10 Cell line:

B16F10 cell line is a high metastatic variant of the murine B16 mouse melanoma, from C57BL/6 (H-2b) mouse strain origin. This cell line is a mixture of both, adherent spindle and epithelial-like cells. B16F10 cell line is a well-established model for metastasis and it is applicable for the study of experimental cancer therapies.
Melanoma tumors express different tumor-associated antigens, which usually induce weak immune responses of short duration. Expression of both tumor-associated antigens p53 and TRP2 by melanoma cells raises the possibility of simultaneously targeting more than one antigen in a therapeutic vaccine.


About turboFP602 protein:

TurboFP602 protein is a red shifted variant of the red fluorescent protein TurboRFP from sea anemone Entacmaea quadricolor [Merzlyak et al., 2007]. It possesses true-red fluorescence (excitation/ emission max = 574/ 602 nm) that is optimal for detection via most popular filter sets. TurboFP602 exhibits fast maturation and also high pH stability. TurboFP602 is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.

  • Reference: P20321
  • Size/Quantity: 3x106 cells / vial
  • Resistance Gene: Puromycin
  • Red Fluorescent Protein: tFP602 (excitation/emission maxima at 574/602 nm, respectively)
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice