_β-ArNOMAD CCK2 Cholecystokinin Receptor Cell Line

Reference: P70808

9.995,00€

_β-ArNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and β-arrestin Nomad Biosensor to GPCR β-arrestin modulation. Each vial of _β-ArNOMAD CCK2 Cholecystokinin Receptor Cell Line contains U2OS cells stably expressing the following constructs:

human CCK2 Cholecystokinin Receptor with no tag
β-arrestin Nomad Biosensor (Green Fluorescence)

_β-ArNOMAD CCK2 Cholecystokinin Receptor Cell Line allows to assay compounds analyzing β-arrestin recruitment. When an agonist binds to CCK2R a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. Due to the presence of ß-Arrestin Nomad Biosensor, this recruitment leads to an increase of green fluorescence intensity. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×10⁶ cells / vial

CCK receptors significantly influence neurotransmission in the brain, regulating anxiety, feeding, and locomotion. Two GPCRs known as CCK1 and CCK2 receptors composes the CCK receptors family. CCK2R gene encodes a G protein-coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. Cholecystokinin B receptor is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is present principally in the central nervous system and the gastrointestinal tract. CCK-B expression may correlate parallel to anxiety and depression phenotypes in humans. _β-ArNOMAD CCK2 Cholecystokinin Receptor Cell Line from Innoprot allows the measurement of the activity and potency of ligands; such as CCK.

Click HERE to know more about CCK2 Cholecystokinin Receptor.