_MPXNOMAD OXYTOCIN Receptor Cell Line

Reference: P70725

19.950,00€

_MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of _MPXNOMAD Oxytocin Receptor Cell Line contains HEK293 cells stably expressing the following constructs:

human oxytovin receptor with no tag
Calcium Nomad Biosensor (Green Fluorescence)
β-arrestin Nomad Biosensor (Red Fluorescence)

_MPXNOMAD Oxytocin Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by calcium flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to OT receptor a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and calcium biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×10⁶ cells / vial

Oxytocin receptors are a type of G-protein-coupled receptor (GPCR) that bind to the hormone oxytocin. Oxytocin receptors are essential for mediating the effects of oxytocin on various physiological and behavioral processes related to social bonding, reproduction, and emotional regulation. Research into oxytocin signaling continues to shed light on its complex role in human biology and behavior. Oxytocin receptors appear in various tissues throughout the body, including the brain, uterus, mammary glands, and other organs. When oxytocin binds to its receptors, it triggers a cascade of intracellular signaling events that ultimately influence physiological and behavioral responses. In the brain, oxytocin signaling plays role with trust, empathy, social recognition, pair bonding, and maternal behavior. _MPXNOMAD CB1 Oxytocin Receptor Cell Line from Innoprot allows the measurement of the activity and potency of ligands; such as oxytocin.

More information about OT Receptor.

Reference: P70725
Nomad Biosensors: β-arrestin & Calcium
NCBI Reference Sequence: NP_000907.2
Size/Quantity: 2 vials containing 3x10⁶ cells / vial
Product Use: For research use only
Shipping Conditions: Dry Ice

Nomad Biosensors: A New Multiplexed Technology for the Screening of GPCR Ligands

Data Sheet

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