Amyotrophic lateral sclerosis (ALS) is one of the most common degenerative disease of the motor neuron system. The TDP-43 pathology seems to be a dominant type of pathology across sporadic ALS types. Our TDP-43 Stress Granules Assay allows the quantification of pathological TDP43 globs into the nucleus and cytosol. This model also permits to monitor…
FUS/TLS Stress Granules Assay from Innoprot requires a stable cell line expressing fluorescent FUS/TLS. During the assay, we incubate cells with test compounds and Sodium Arsenite to induce the FUS/TLS aggregation. Finally, we quantify fluorescent FUS/TLS nuclear globs using the BD Pathway HCS Reader and Attovision Compartimentalization Software. Amyotrophic lateral sclerosis (ALS) is one of…
A brief exposure to glutamate in vitro causes neuronal death, which makes glutamate a useful tool to evaluate neuroprotective activities. Innoprot excitotoxicity in vitro assay offers the advantage of a rapid analysis of the neuroprotective potential of a molecule. In our assay, we incubate rat primary neurons with test compounds 24 hours before L-glutamate insult.…