Thyroid-stimulating hormone (also known as thyrotropin, thyrotropic hormone or TSH) is a pituitary hormone that stimulates the thyroid gland. Pituitary gland produces and releases TSH into the bloodstream. It controls production of the thyroid hormones, thyroxine and triiodothyronine, by the thyroid gland by binding to receptors located on cells in the thyroid gland. Thyroxine and triiodothyronine are essential to maintaining the body’s metabolic rate, heart and digestive functions, muscle control, and brain development. TSH Functional Assay from Innoprot allows the measurement of the activity and potency of recombinant human TSH (rhTSH).
To analyze the activity of rhTSH, we use a stable cell line stably expressing Thyroid-stimulating hormone receptor (TSHR). This cell line also expresses the cAMP-Nomad biosensor, which increases its fluorescence intensity upon TSHR activation. When rhTSH binds to the TSHR, it activates a G protein, which in turn triggers a cellular response mediated by second messengers (cAMP). This cAMP-Nomad TSHR cell line measures cAMP increase in the cytosol in a dose-response way in presence of rhTSH.
Cell line used: cAMP-Nomad TSHR Cell Line
Readout: cAMP increase
EC50 Agonist: 3.27 × 10-8 gr/ml
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry