Endothelial cells constitute the natural interface between the blood and the underlying tissue. Changes in endothelial cell function appear to play a key role in the pathogenesis of atherosclerosis. Endothelial cells synthesize and secrete activators as well as inhibitors of both the coagulation system and the fibrinolysis system in addition to mediators that influence the adhesion and aggregation of blood platelets. Endothelial cells also release molecules that control cell proliferation and modulate vessel wall tone. Human aortic endothelial cells (HAEC) produce antithrombotic and thrombotic factors such as t-PA and PAI-1. They respond to TNFalpha by modifying growth characteristics, producing cytokines such as GM-CSF. Green Fluorescent Immortalized Human Aortic Endothelial Cells allow also to stablish in vitro models for HIgh Throughput Screening and High Content Screening.
About turboGFP protein:
tGFP is an improved variant of the green fluorescent protein CopGFP from copepoda Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins. TurboGFP is useful for applications where fast appearance of bright fluorescence is crucial. It is also useful for cell and organelle labeling and tracking the promoter activity.