Hepatocyte Medium (HM) is a complete medium designed for optimal growth of normal human hepatocytes in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes growth of normal human and animal hepatocytes in vitro.
Hepatocyte Medium Kit is a complete medium designed for optimal growth of normal primary hepatocytes in vitro. Each Hepatocyte Medium Kit consists of the next components:
- 500 ml of Hepatocyte basal medium
- 25 ml of fetal bovine serum
- 5 ml of Hepatocyte Growth supplement
- 5 ml of penicillin/streptomycin solution.