Human Myometrial microvascular endothelial cells (HMMEC) are located in the myometrium between the endometrium and the serosa of the uterus. They exhibit typical cobblestone morphology. During pregnancy, angiogenesis occurs through MMEC proliferation in order to support the hypertrophy of the myometrial smooth muscle cell layer. Significant evidence also indicates that endothelial cell dysfunction in the myometrium may contribute to the development of preeclampsia. In addition, MMEC proliferation and angiogenesis may play a role in the formation of leiomyomata. Studies have shown that MMEC express the OTR receptor, which may promote vasodilation in the microvasculature of the myometrium. Human MMEC are a useful in vitro model to elucidate the mechanisms of angiogenesis and to develop treatments for female reproductive tract disorders. Human Myometrial Microvascular Endothelial Cells are also useful tools to develop disease models for High Throughput and High Content Screening.