The seminal vesicles (SV) are a pair of tubular glands located near the prostate. They are essential to the urinary system. They function under androgen control to produce and secrete fluid into the ejaculatory duct. The SV consists of three layers: the inner basal mucosal layer composed of simple cuboidal and pseudo-stratified columnar epithelial cells, the middle muscular layer formed by smooth muscle cells, and finally the outer layer made up of dense connective tissue. Various pathological conditions can arise in the SV, including congenital SV cysts, seminal vesculitis, and primary and secondary neoplasms. Additionally, SV invasion is often a prognostic marker in prostate cancer. SV fibroblasts offer unique opportunities to study many features of the SV. Human Seminal Vesicle Fibroblasts are also useful tools to develop disease models for High Throughput and High Content Screening.