Sertoli Cell Medium Kit is a complete medium designed for optimal growth of normal primary Sertoli Cells in vitro. Each Sertoli Cell Medium Kit consists of the next components:
500 ml of Sertoli Cell basal medium
25 ml of fetal bovine serum
5 ml of Sertoli Cell Growth supplement
5 ml of penicillin/streptomycin solution.
Sertoli Cell Medium (SerCM) is a complete medium designed for optimal growth of normal human sertoli cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human sertoli cells in vitro.
Reference: P60139
Size/Quantity: 500 ml
Storage: Store the basal medium at 4°C, the SerCGS, the FBS and the P/S solution at -20°C. Protect from light.