_MPXNOMAD NTS1 Neurotensin Receptor Cell Line

_MPXNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of _MPXNOMAD NTS1 Neurotensin Receptor Cell Line contains U2OS cells stably expressing the following constructs:

• human NTS1 Neurotensin Receptor with no tag
• Calcium Nomad Biosensor (Green Fluorescence)
• β-arrestin Nomad Biosensor (Red Fluorescence)

_MPXNOMAD NTS1 Neurotensin Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca²⁺ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to NTS1R a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca²⁺ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×10⁶ cells / vial