_cAMPNOMAD ADRB2 Receptor Cell Line

_cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of _cAMPNOMAD ADRB2 Receptor Cell Line contains U2OS cells stably expressing the following constructs:

• human B₂ adrenoceptor with no tag
• cAMP Nomad Biosensor (Red Fluorescence)

_cAMPNOMAD B₂ adrenoceptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by cAMP involving receptor activation. When an agonist binds to ADRB2 a G protein is activated which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×10⁶ cells / vial