_DAGNOMAD Muscarinic Acetylcholine Receptor M5 Cell Line

_DAGNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and diacylglycerol (DAG) Nomad Biosensor to monitor G-protein modulation by DAG release. Each vial of _DAGNOMAD Muscarinic M5 Receptor Cell Line contains U2OS cells stably expressing the following constructs:

• Human Muscarinic M5 Receptor (M5R) with no tag
• DAG Nomad Biosensor (Red Fluorescence)

_DAGNOMAD Muscarinic M5 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by G_q/G₁₁ involving receptor activation. When an agonist binds to M5 Receptor, the G_q/G₁₁ protein is activated which, in turn, triggers a cellular response mediated by DAG via Phospholipase C stimulation. This leads to an increase of red fluorescence intensity of DAG biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×10⁶ cells / vial