Immortalized Human Dermal Fibroblasts

Reference: P10858-IM

2.085,00€

Immortalized Human Dermal Fibroblasts (IM-HDF) provided by Innoprot have been developed by immortalizing primary human healthy dermal fibroblasts with hTERT or SV40T.

Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 4th passage while the tranduced cells go beyond 30 passages.

Each vial of Immortalized Human Dermal Fibroblasts contains more than 1 million viable cells. Innoprot also offers optimized medium and reagents for the growth of IM-HDF which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro IM-HDF culture.

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Cultures of immortalized human dermal fibroblasts are very useful for a wide range of cellular and molecular studies. This is mainly because fibroblasts are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. There is evidence showing that fibroblasts in different organs are intrinsically different. Dermal fibroblasts also secrete large quantities of hyaluronan in response to inflammatory stimuli. During wound healing, human dermal fibroblasts switch from a migratory, repopulating phenotype to a contractile, matrix-reassembling phenotype. Immortalized Human Dermal Fibroblasts also allow to stablish in vitro models for HIgh Throughput and High Content Screening. More information about Dermal Fibroblasts Interested in Cell-damage assays using Dermal Fibroblasts?