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Gamma secretase activity assay

Alzheimer's Disease, Alzheimer's Disease, CNS, Disease Models, Phenotypic Assays

Gamma secretase activity assay from Innoprot allows the monitoring of γ-secretase proteolytic process in living cells. Assay is performed on a cell line expressing a fluorescent construct (APP-C99) containing the γ-secretase cleaving site. When γ-secretase activity is inhibited by any antagonist, fluorescent aggregates appear in the cell cytoplasm in a dose-response manner.

Cell line used:APP-C99-tGFP U2OS

Readout: Fluorescent APP-C99 aggregates quantification

Antagonist: DAPT
EC50 Antagonist: 1.10 x 10<sup>-7</sup> M
Type of Assay: Cell-based / Functional
Detection Method: Image Analysis

Assay: Alzheimer's Disease, Alzheimer's Disease, CNS, Disease Models, Phenotypic Assays

Area: Alzheimer's Disease, CNS

Type: Alzheimer's Disease in vitro models, In vitro Disease Models - Phenotypic Assays

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